Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.
Part I Principles and Instrumental
1. The CE-Way of Thinking: "All is Relative!”
Philippe Schmitt-Kopplin and Agnes Fekete
2. A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic
Behaviors of Small Molecules in Free Zone Electrophoresis
Philippe Schmitt-Kopplin and Agnes Fekete<
3. Derivatization in Capillary Electrophoresis
M. Luisa Marina and María Castro-Puyana
4. Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS
Peptide Analysis-Strategies and Implementation
Martin Pattky, Katalin Barkovits, Oliver Weiergräber, and Carolin Huhn
5. Micellar Electrokinetic Chromatography of Aminoglycosides
Ulrike Holzgrabe, Stefanie Schmitt, and Frank Wienen
6. Microemulsion Electrokinetic Chromatography
Wolfgang Buchberger
7. Non Aqueous Capillary Electrophoresis Mass Spectrometry
Christian W. Klampfl and Markus Himmelsbach
8. Ionic Liquids in Capillary Electrophoresis
Ulrike Holzgrabe and Joachim Wahl
9. CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve
Felix J. Kohl and Christian Neusüß
10. Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry
Bernhard Michalke
11. Use of CE to Analyze Solutes in Pico- and Nano-Litre Samples from Plant Cells and
Rhizosphere
A. Deri Tomos
Part II Applications from Small to Macromolecules
12. Analysis of Small Ions with Capillary Electrophoresis
Jatinder Singh Aulakh, Ramandeep Kaur, and Ashok Kumar Malik
13. Metal Ions Analysis with Capillary Zone Electrophoresis
Ashok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur
14. Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis
Daniela Fico, Antonio Pennetta, and Giuseppe E. De Benedetto
15. Enantiomer Separations by Capillary Electrophoresis
Gerhard K. E. Scriba, Henrik Harnisch, and Qingfu Zhu
16. Capillary Electrophoresis of Mono- and Oligosaccharides
Mila Toppazzini, Anna Coslovi, Marco Rossi, Anna Flamigni, Edi Baiutti, and Cristiana Campa
17. Use of Capillary Electrophoresis for Polysaccharide Studies and Applications
Amelia Gamini, Anna Coslovi, Mila Toppazzini, Isabella Rustighi, Cristiana Campa, Amedeo Vetere,
and Sergio Paoletti
18. Separation of Peptides by Capillary Electrophoresis
Gerhard K. E. Scriba
19. Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae
sing Capillary Electrophoresis Approach
Pawel Pomastowski, Viorica Railean-Plugaru, and Boguslaw Buszewski
20. Capillary Electrophoretic Analysis of Classical Organic Pollutants
Ashok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur
21. Capillary Electrophoresis in Metabolomics
Tanja Verena Maier and Philippe Schmitt-Kopplin
22. Capillary Electrophoresis in Food and Foodomics
Clara Ibáñez, Tanize Acunha, Alberto Valdés, Virginia García-Cañas, Alejandro Cifuentes, and
Carolina Simó
23. Capillary Electrophoresis in Wine Science
Christian Coelho, Franck Bagala, Régis Gougeon, and Philippe Schmitt-Kopplin
This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.
This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.
The CE-Way of Thinking: "All is Relative!".- A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis.- Derivatization in Capillary Electrophoresis.- Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis-Strategies and Implementation.- Micellar Electrokinetic Chromatography of Aminoglycosides.- Microemulsion Electrokinetic Chromatography.- Non Aqueous Capillary Electrophoresis Mass Spectrometry.- Ionic Liquids in Capillary Electrophoresis.- CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve.- Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry.- Use of CE to Analyze Solutes in Pico- and Nano-Litre Samples from Plant Cells and Rhizosphere.- Analysis of Small Ions with Capillary Electrophoresis.- Metal Ions Analysis with Capillary Zone Electrophoresis.- Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis.- Enantiomer Separations by Capillary Electrophoresis.- Capillary Electrophoresis of Mono- and Oligosaccharides.- Use of Capillary Electrophoresis for Polysaccharide Studies and Applications.- Separation of Peptides by Capillary Electrophoresis.- Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach.- Capillary Electrophoretic Analysis of Classical Organic Pollutants.- Capillary Electrophoresis in Metabolomics.- Capillary Electrophoresis in Food and Foodomics.- Capillary Electrophoresis in Wine Science.
Inhaltsverzeichnis
Part I Principles and Instrumental
1. The CE-Way of Thinking: "All is Relative!"
Philippe Schmitt-Kopplin and Agnes Fekete
2. A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic
Behaviors of Small Molecules in Free Zone Electrophoresis
Philippe Schmitt-Kopplin and Agnes Fekete<
3. Derivatization in Capillary Electrophoresis
M. Luisa Marina and María Castro-Puyana
4. Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS
Peptide Analysis-Strategies and Implementation
Martin Pattky, Katalin Barkovits, Oliver Weiergräber, and Carolin Huhn
5. Micellar Electrokinetic Chromatography of Aminoglycosides
Ulrike Holzgrabe, Stefanie Schmitt, and Frank Wienen
6. Microemulsion Electrokinetic Chromatography
Wolfgang Buchberger
7. Non Aqueous Capillary Electrophoresis Mass Spectrometry
Christian W. Klampfl and Markus Himmelsbach
8. Ionic Liquids in Capillary Electrophoresis
Ulrike Holzgrabe and Joachim Wahl
9. CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve
Felix J. Kohl and Christian Neusüß
10. Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry
Bernhard Michalke
11. Use of CE to Analyze Solutes in Pico- and Nano-Litre Samples from Plant Cells and
Rhizosphere
A. Deri Tomos
Part II Applications from Small to Macromolecules
12. Analysis of Small Ions with Capillary Electrophoresis
Jatinder Singh Aulakh, Ramandeep Kaur, and Ashok Kumar Malik
13. Metal Ions Analysis with Capillary Zone Electrophoresis
Ashok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur
14. Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis
Daniela Fico, Antonio Pennetta, and Giuseppe E. De Benedetto
15. Enantiomer Separations by Capillary Electrophoresis
Gerhard K. E. Scriba, Henrik Harnisch, and Qingfu Zhu
16. Capillary Electrophoresis of Mono- and Oligosaccharides
Mila Toppazzini, Anna Coslovi, Marco Rossi, Anna Flamigni, Edi Baiutti, and Cristiana Campa
17. Use of Capillary Electrophoresis for Polysaccharide Studies and Applications
Amelia Gamini, Anna Coslovi, Mila Toppazzini, Isabella Rustighi, Cristiana Campa, Amedeo Vetere,
and Sergio Paoletti
18. Separation of Peptides by Capillary Electrophoresis
Gerhard K. E. Scriba
19. Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae
sing Capillary Electrophoresis Approach
Pawel Pomastowski, Viorica Railean-Plugaru, and Boguslaw Buszewski
20. Capillary Electrophoretic Analysis of Classical Organic Pollutants
Ashok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur
21. Capillary Electrophoresis in Metabolomics
Tanja Verena Maier and Philippe Schmitt-Kopplin
22. Capillary Electrophoresis in Food and Foodomics
Clara Ibáñez, Tanize Acunha, Alberto Valdés, Virginia García-Cañas, Alejandro Cifuentes, and
Carolina Simó
23. Capillary Electrophoresis in Wine Science
Christian Coelho, Franck Bagala, Régis Gougeon, and Philippe Schmitt-Kopplin
Klappentext
This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nnCutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.nnnn
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
