Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will be usefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.
PartI: Background
1.ER to Golgi Dependent Protein Secretion: The Conventional Pathway
CorradoViotti
2. UnconventionalProtein Secretion in Animal Cells
Fanny Ng and Bor Luen Tang
3. UnconventionalProtein Secretion in Plants
Destiny J. Davis,Byung-Ho Kang, Angelo S. Heringer,Thomas E. Wilkop, and Georgia Drakakaki
Part II: UPSContemplates Multidisciplinary Approaches for Plants
4. ChemicalSecretory Pathway Modulation in Plant Protoplasts
FrancescaDe Marchis, Andrea Pompa, and Michele Bellucci
5.From Cytosol to the Apoplast: The HygromycinPhosphotransferase (HYGR) Model in Arabidopsis
Haiyan Zhang and Jinjin Li
6.Following the Time-Course of Post-Pollination Events by Transmission ElectronMicroscopy (TEM): Buildup of Exosome-Like Structures with CompatiblePollinations
DaryaSafavian, Jennifer Doucet, and Daphne R. Goring
PartIII: UPS ContemplatesMultidisciplinary Approaches for Animals and Fungi
7.Investigating AlternativeTransport of Integral Plasma Membrane Proteins from the ER to the Golgi:Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
MargaridaD. Amaral, Carlos M. Farinha, Paulo Matos, and Hugo M. Botelho
8.Quantification of a Non-Conventional Protein Secretion:The Low Molecular Weight FGF-2 Example
Tania Arcondéguy, Christian Touriol, and EricLacazette
9. HumanPrimary Keratinocytes as a Tool for the Analysis of Caspase-1-DependentUnconventional Protein Secretion
GerhardE. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi,Hans-Dietmar Beer
10.A Reporter System to Study Unconventional Secretion of Proteins Avoiding
N-glycosylation in
Ustilago maydisJanpeter Stock, Marius Terfrüchte, andKerstin Schipper
11.Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis AndQuantification
MarcosVinicios Salles Dias, Vilma Regina Martins, and Glaucia Noeli Maroso Hajj
12.Analysis of Yeast Extracellular Vesicles
Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas,Wendell Girard-Dias, Anderson J.Franzen, Susana Frasés, Kildare Miranda, and Leonardo Nimrichter
13. Exploring the LeishmaniaHydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live CellImaging Methods
LornaMacLean, Helen Price, and Peter O´Toole
14. Characterization of the Unconventional Secretion of the Ebola MatrixProtein VP40
Olivier Reynard and Mathieu Mateo
15. Role andCharacterization of synuclein-? Unconventional Protein Secretion in CancerCells
CaiyunLiu, Like Qu, and Chengchao Shou
PartIV: SecretomeIsolation from Plant and Animal Samples to Identify Leaderless SecretoryProteins (LSP)
16.Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF)
PavelGromov and Irina Gromova
17.Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction inGrapevine
BertrandDelaunois, Fabienne Baillieul, Christophe Clément, Philippe Jeandet,and Sylvain Cordelier
18. Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation onSucrose/Deuterium Oxide (D2O) Density Cushions
Christopher Stanly, Immacolata Fiume,Giovambattista Capasso, and Gabriella Pocsfalvi
This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will be usefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.
This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format,chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will beusefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.
ER to Golgi Dependent Protein Secretion: The Conventional Pathway.- UnconventionalProtein Secretion in Animal Cells.- UnconventionalProtein Secretion in Plants.- ChemicalSecretory Pathway Modulation in Plant Protoplasts.- From Cytosol to the Apoplast: The HygromycinPhosphotransferase (HYGR) Model in Arabidopsis.- Following the Time-Course of Post-Pollination Events by Transmission ElectronMicroscopy (TEM): Buildup of Exosome-Like Structures with CompatiblePollinations.- Investigating AlternativeTransport of Integral Plasma Membrane Proteins from the ER to the Golgi:Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).- Quantification of a Non-Conventional Protein Secretion:The Low Molecular Weight FGF-2 Example.- HumanPrimary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent.- A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-glycosylation in Ustilago maydis.- Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis AndQuantification.- Analysis of Yeast Extracellular Vesicles.- Exploring the LeishmaniaHydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live CellImaging Methods.- Characterization of the Unconventional Secretion of the Ebola MatrixProtein VP40.- Role andCharacterization of synuclein-Gamma Unconventional Protein Secretion in CancerCells.- Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF).- Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction inGrapevin.- Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation onSucrose/Deuterium Oxide (D2O) Density Cushions.
Inhaltsverzeichnis
Part
I: Background
1.
ER to Golgi Dependent Protein Secretion: The Conventional Pathway
Corrado
Viotti
2. Unconventional
Protein Secretion in Animal Cells
Fanny Ng and Bor Luen Tang
3. Unconventional
Protein Secretion in Plants
Destiny J. Davis,
Byung-Ho Kang, Angelo S. Heringer,
Thomas E. Wilkop, and Georgia Drakakaki
Part II: UPS
Contemplates Multidisciplinary Approaches for Plants
4. Chemical
Secretory Pathway Modulation in Plant Protoplasts
Francesca
De Marchis, Andrea Pompa, and Michele Bellucci
5.
From Cytosol to the Apoplast: The Hygromycin
Phosphotransferase (HYGR) Model in Arabidopsis
Haiyan Zhang and Jinjin Li
6.
Following the Time-Course of Post-Pollination Events by Transmission Electron
Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible
Pollinations
Darya
Safavian, Jennifer Doucet, and Daphne R.
Goring
Part
III: UPS Contemplates
Multidisciplinary Approaches for Animals and Fungi
7.
Investigating Alternative
Transport of Integral Plasma Membrane Proteins from the ER to the Golgi:
Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Margarida
D. Amaral, Carlos M. Farinha, Paulo Matos, and Hugo M. Botelho
8.
Quantification of a Non-Conventional Protein Secretion:
The Low Molecular Weight FGF-2 Example
Tania Arcondéguy, Christian Touriol, and Eric
Lacazette
9. Human
Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent
Unconventional Protein Secretion
Gerhard
E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi,
Hans-Dietmar Beer
10.
A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-glycosylation in Ustilago maydis
Janpeter Stock, Marius Terfrüchte, and
Kerstin Schipper
11.
Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis And
Quantification
Marcos
Vinicios Salles Dias, Vilma Regina Martins, and Glaucia Noeli Maroso Hajj
12.
Analysis of Yeast Extracellular Vesicles
Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas,
Wendell Girard-Dias, Anderson J.
Franzen, Susana Frasés, Kildare Miranda, and Leonardo Nimrichter
13. Exploring the Leishmania
Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell
Imaging Methods
Lorna
MacLean, Helen Price, and Peter O'Toole
14. Characterization of the Unconventional Secretion of the Ebola Matrix
Protein VP40
Olivier Reynard and Mathieu Mateo
15. Role and
Characterization of synuclein-¿ Unconventional Protein Secretion in Cancer
Cells
Caiyun
Liu, Like Qu, and Chengchao Shou
Part
IV: Secretome
Isolation from Plant and Animal Samples to Identify Leaderless Secretory
Proteins (LSP)
16.
Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF)
Pavel
Gromov and Irina Gromova
17.
Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in
Grapevine
Bertrand
Delaunois, Fabienne Baillieul, Christophe Clément, Philippe Jeandet,
and Sylvain Cordelier
18. Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on
Sucrose/Deuterium Oxide (D2O) Density Cushions
Christopher Stanly, Immacolata Fiume,
Giovambattista Capasso, and Gabriella Pocsfalvi
Klappentext
This volume presents relevant
background information to understanding the molecular basis governing
unconventional protein secretion (UPS), and in particular explores the latest
techniques and protocols that have been successfully applied for the study of
this topic. Detailed chapters include an overview of conventional and
unconventional secretory pathways along with multidisciplinary approaches and
methods used for UPS analysis in different organisms. Written in the highly successful Methods in
Molecular Biology series format,chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and cutting-edge, Unconventional
Protein Secretion: Methods and Protocols will beuseful
for all interested in the secretory pathway field as well as applications in
cell biology, cell development, biomedical research, and healthcare.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
