Provides an easily accessible reference volume for cell-based phenotypic screening written by leading researchers in the field
Details the process of turning a biological observation into a high-throughput screen
Covers a wide range of cell-based assay formats and systems
Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, and approaches to data analysis
Comprehensive guide to developing, running, and analyzing cell-based assays in high-throughput screening
Provides an easily accessible reference volume for cell-based phenotypic screening written by leading researchers in the field
Details the process of turning a biological observation into a high-throughput screen
Covers a wide range of cell-based assay formats and systems
Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, and approaches to data analysis
Comprehensive guide to developing, running, and analyzing cell-based assays in high-throughput screening
Includes supplementary material: sn.pub/extras
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
Chapter 1: Introduction: Cell-based Assays for High-throughput Screening W. Frank An and Nicola J. Tolliday Chapter 2: High-throughput Screening of Model Bacteria Soumaya Zlitni, Jan E. Blanchard, and Eric D. Brown Chapter 3: Cell-based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells Michael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Toebben, Nathalie Aulner, and Thomas Mayer Chapter 4: Large-scale Small-molecule Screen Using Zebrafish Embryos Charles C. Hong Chapter 5: Whole-animal High-throughput Screens: The C. elegans Model Eyleen J. O´Rourke, Annie L. Conery, and Terence I. Moy Chapter 6: Whole-organism Screening: Plants April Agee and David Carter Chapter 7: Fluorescence-based Assays W. Frank An Chapter 8: Reporter-gene Assays Andy M. F. Liu, David C. New, Rico K. H. Lo, and Yung H. Wong Chapter 9: Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-restoration Assay Aruna D. Balgi and Michel Roberge Chapter 10: Assay for Isolation of Inhibitors of Her2-kinase Expression Gabriela Chiosis and Adam B. Keeton Chapter 11: ImageJ: A Primer Katherine Luby-Phelps Chapter 12: High-content Screening: Flow Cytometry Analysis Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, and Larry A. Sklar Chapter 13: High-throughput Real-time PCR for Detection of Gene-expression Levels Bridget K. Wagner and Zoltan Arany Chapter 14: Interpretation of Uniform-well Readouts Serene Josiah Chapter 15: Extracting Rich Information from Images Anne E. Carpenter
From the reviews:
"`Cell-based assays for high-throughput screening´ is a highly practical resource, written by experts in each of the techniques ... . very useful from a practical point of view, and anyone looking to find a new assay system will find a wealth of ideas here. ... aimed at molecular biologists, many other scientists - such as natural product chemists looking for new lead molecules - should find it equally useful." (Elizabeth Williamson, Phytotherapy Research, September, 2009)
As the use of high-throughput screening expands, the need for detailed reference materials becomes ever more pressing. This book fills an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening.
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology(TM) series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
Introduction: Cell-Based Assays for High-Throughput Screening.- High-Throughput Screening of Model Bacteria.- Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells.- Large-Scale Small-Molecule Screen Using Zebrafish Embryos.- Whole-Animal High-Throughput Screens: The C. elegans Model.- Whole-Organism Screening: Plants.- Fluorescence-Based Assays.- Reporter Gene Assays.- Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-Restoration Assay.- Assay for Isolation of Inhibitors of Her2-Kinase Expression.- High-Content Screening: Flow Cytometry Analysis.- High-Throughput Real-Time PCR for Detection of Gene-Expression Levels.- Interpretation of Uniform-Well Readouts.- Extracting Rich Information from Images.
From the reviews:
"'Cell-based assays for high-throughput screening' is a highly practical resource, written by experts in each of the techniques ... . very useful from a practical point of view, and anyone looking to find a new assay system will find a wealth of ideas here. ... aimed at molecular biologists, many other scientists - such as natural product chemists looking for new lead molecules - should find it equally useful." (Elizabeth Williamson, Phytotherapy Research, September, 2009)
Inhaltsverzeichnis
Chapter 1: Introduction: Cell-based Assays for High-throughput Screening W. Frank An and Nicola J. Tolliday Chapter 2: High-throughput Screening of Model Bacteria Soumaya Zlitni, Jan E. Blanchard, and Eric D. Brown Chapter 3: Cell-based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells Michael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Toebben, Nathalie Aulner, and Thomas Mayer Chapter 4: Large-scale Small-molecule Screen Using Zebrafish Embryos Charles C. Hong Chapter 5: Whole-animal High-throughput Screens: The C. elegans Model Eyleen J. O¿Rourke, Annie L. Conery, and Terence I. Moy Chapter 6: Whole-organism Screening: Plants April Agee and David Carter Chapter 7: Fluorescence-based Assays W. Frank An Chapter 8: Reporter-gene Assays Andy M. F. Liu, David C. New, Rico K. H. Lo, and Yung H. Wong Chapter 9: Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-restoration Assay Aruna D. Balgi and Michel Roberge Chapter 10: Assay for Isolation of Inhibitors of Her2-kinase Expression Gabriela Chiosis and Adam B. Keeton Chapter 11: ImageJ: A Primer Katherine Luby-Phelps Chapter 12: High-content Screening: Flow Cytometry Analysis Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, and Larry A. Sklar Chapter 13: High-throughput Real-time PCR for Detection of Gene-expression Levels Bridget K. Wagner and Zoltan Arany Chapter 14: Interpretation of Uniform-well Readouts Serene Josiah Chapter 15: Extracting Rich Information from Images Anne E. Carpenter
Klappentext
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology¿ series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.nnCutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
Provides an easily accessible reference volume for cell-based phenotypic screening written by leading researchers in the field
Details the process of turning a biological observation into a high-throughput screen
Covers a wide range of cell-based assay formats and systems
Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, and approaches to data analysis
Comprehensive guide to developing, running, and analyzing cell-based assays in high-throughput screening
Includes supplementary material: sn.pub/extras
