New and novel techniques in biological aging research
Cutting-edge applications of proteomics and metabolomics
Identifying genes that extend lifespan using high-throughput screening systems
This book investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions.
|
Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. The editors have carefully selected only those topics that are considered mainstays of the field or are showing promise in revolutionizing this relatively new science. The three main areas of focus in this cutting-edge compendium of biological aging research are: methods that are basic to understanding the fundamental mechanisms of cellular aging; techniques used to intervene in the aging process; and approaches to analyzing the many molecular processes of biological aging. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions in the exciting and expanding field of biological aging.
Contents:PrefaceContributors Techniques for Analysis of Biological Aging Trygve O. Tollefsbol Aging Cell Culture: Methods and Observations Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrews, and Trygve O. Tollefsbol Methods to Detect Biomarkers of Cellular Senescence: The Senescence Associated b-galactosidase Koji Itahana, Judith Campisi, and Goberdhan P. Dimri Methods of Cell Sorting of Young and Senescent Cells Joao F. Passos and Thomas von Zglinicki Telomere Length Analysis Andres Canela, Peter Klatt, and Maria A. Blasco A Method to Detect DNA Methyltransferase I Gene Tranillegalscription In Vitro in Aging Systems Joel B. Berletch, Lucy G. Andrews, and Trygve O. Tollefsbol A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro Joel B. Berletch, Sharla M. O. Phipps, Sabrina L. Walthall, Lucy G. Andrews, and Trygve O. Tollefsbol The Chronological Life Span of Saccharomyces Cerevisiae Paola Fabrizio and Valter D. Longo Caloric Restriction and Life-Span Determination of Yeast Cells Oliver Medvedik and David A. Sinclair Methods of Nutrigenomics and Longevity Studies in Drosophila: Effects of Diets High in Sucrose, Palmitic Acid, Soy or Beef Jiatao Ye, Xiangqin Cui, Ann Loraine, Kerry Bynum, Nam Chul Kim, Gregory White, Maria De Luca, Mark D. Garfinkel, Xiangyi Lu, and Douglas M. Ruden Caloric Restriction Mimetics: A Novel Approach for Biogerontology Mark A. Lane, George S. Roth, and Donald K. Ingram Extension of Cell Life-Span Using Exogenous Telomerase Mo K. Kang and No-Hee Park Inducing Cellular Senescence Using Defined Genetic Elements Hiroshi Nakagawa and Oliver G. Opitz Methods of Induction of Cellular Senescence Using Oxidative Stresslas Hales Nuclear Transfer Methods to Study Aging Lin Liu and David L. Keefe Use of Ribozymes in Cellular Aging Research Custer C. Deocaris, Sunil C. Kaul, Kazunari Taira, and Renu Wadhwa Methods of Evaluation of Pharmacological Drugs Effects on Aging and Life Span in Mice Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski Identifying Genes that Extend Life-Span Using a High Throughput Screening System Cuiying Chen and Roland Contreras Application of DNA Microarray Technology to Gerontological Studies Sergey V. Anisimov Detection and Analysis of Somatic Mutations at a LacZ Reporter Locus in Higher Organisms: Application to Mus Musculus and Drosophila Melanogaster Ana Maria Garcia, Rita A. Busuttil, Armando Rodriguez, Carlos Cabrera, Martha Lundell, Martijn E. T. Dolle, and Jan Vijg Subtractive Hybridization Techniques to Study Cellular Senescence Michal B. Sternberg and Shimon Gepstein Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity Maria De Luca and Jeff Leips Quantitative Trait Locus (QTL) Mapping in Aging Systems Hui-Chen Hsu, Lu Lu, Nengjun Yi, Gary Van Zant, Robert W. Williams, and John D. Mountz 2D Gel Proteomics: An Approach to Study Age-related Differences in Protein-abundance or -Isoform Complexity in Biological Samples Helen Kim, Shannon Eliuk, Jessy Deshane, Sreelatha Meleth, Todd Sanderson, Anita Pinner, Gloria Robinson, Landon Wilson, Marion Kirk, and Stephen Barnes Metabolomics in the Study of Aging and Caloric Restriction Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah- Daouk, and Wayne R. MatsonIndex
From the reviews:
"For research gerontologists, this book is a boon." -Doody's Book Review, Weighted Numerical Score:90 - 4 Stars
"This can be a useful book for biogerontologists. ... Comprised of 25 generally well-written articles ... this book covers various methods and protocols for studying cellular aging of human cells, yeast cells, and mice. ... a welcome source of useful information for researchers intending to find their way into deillegalscriptive, analytical and interventive research in biogerontology." (Suresh Rattan, Biogerontology, Issue 9, 2008)
Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. The editors have carefully selected only those topics that are considered mainstays of the field or are showing promise in revolutionizing this relatively new science. The three main areas of focus in this cutting-edge compendium of biological aging research are: methods that are basic to understanding the fundamental mechanisms of cellular aging; techniques used to intervene in the aging process; and approaches to analyzing the many molecular processes of biological aging. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions in the exciting and expanding field of biological aging.
Techniques for Analysis of Biological Aging.- Aging Cell Culture.- Methods to Detect Biomarkers of Cellular Senescence.- Methods for Cell Sorting of Young and Senescent Cells.- Telomere Length Analysis.- A Method to Detect DNA Methyltransferase I Gene Tranillegalscription In Vitro in Aging Systems.- A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro.- The Chronological Life Span of Saccharomyces cerevisiae.- Caloric Restriction and Life Span Determination of Yeast Cells.- Methods for Nutrigenomics and Longevity Studies in Drosophila.- Caloric Restriction Mimetics.- Extension of Cell Life Span Using Exogenous Telomerase.- Inducing Cellular Senescence Using Defined Genetic Elements.- Methods of Cellular Senescence Induction Using Oxidative Stress.- Nuclear Transfer Methods to Study Aging.- Use of Ribozymes in Cellular Aging Research.- Methods of Evaluating the Effect of Pharmacological Drugs On Aging and Life Span in Mice.- Identifying Genes that Extend Life Span Using a High-Throughput Screening System.- Application of DNA Microarray Technology to Gerontological Studies.- Detection and Analysis of Somatic Mutations at a lacZ Reporter Locus in Higher Organisms.- Subtractive Hybridization Techniques to Study Cellular Senescence.- Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity.- Quantitative Trait Locus (QTL) Mapping in Aging Systems.- 2D Gel Proteomics.- Metabolomics in the Study of Aging and Caloric Restriction.
Inhaltsverzeichnis
Contents:
Preface
Contributors
Techniques for Analysis of Biological Aging
Trygve O. Tollefsbol
Aging Cell Culture: Methods and Observations
Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrews,
and Trygve O. Tollefsbol
Methods to Detect Biomarkers of Cellular Senescence: The Senescence Associated b-galactosidase
Koji Itahana, Judith Campisi, and Goberdhan P. Dimri
Methods of Cell Sorting of Young and Senescent Cells
Joao F. Passos and Thomas von Zglinicki
Telomere Length Analysis
Andres Canela, Peter Klatt, and Maria A. Blasco
A Method to Detect DNA Methyltransferase I Gene Tranillegalscription In Vitro in Aging Systems
Joel B. Berletch, Lucy G. Andrews, and Trygve O.
Tollefsbol
A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro
Joel B. Berletch, Sharla M. O. Phipps, Sabrina L. Walthall, Lucy G. Andrews, and Trygve O. Tollefsbol
The Chronological Life Span of Saccharomyces Cerevisiae
Paola Fabrizio and Valter D. Longo
Caloric Restriction and Life-Span Determination of Yeast Cells
Oliver Medvedik and David A. Sinclair
Methods of Nutrigenomics and Longevity Studies in Drosophila: Effects of Diets High in Sucrose, Palmitic Acid, Soy or Beef
Jiatao Ye, Xiangqin Cui, Ann Loraine, Kerry Bynum, Nam Chul Kim, Gregory White, Maria De Luca, Mark D. Garfinkel, Xiangyi Lu, and Douglas M. Ruden
Caloric Restriction Mimetics: A Novel Approach for Biogerontology
Mark A. Lane, George S. Roth, and Donald K. Ingram
Extension of Cell Life-Span Using Exogenous Telomerase
Mo K. Kang and No-Hee Park
Inducing Cellular Senescence Using Defined Genetic Elements
Hiroshi Nakagawa and Oliver G. Opitz
Methods of Induction of Cellular Senescence Using Oxidative Stresslas Hales
Nuclear Transfer Methods to Study Aging
Lin Liu and David L. Keefe
Use of Ribozymes in Cellular Aging Research
Custer C. Deocaris, Sunil C. Kaul, Kazunari Taira, and Renu Wadhwa
Methods of Evaluation of Pharmacological Drugs Effects on Aging and Life Span in Mice
Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski
Identifying Genes that Extend Life-Span Using a High Throughput Screening System
Cuiying Chen and Roland Contreras
Application of DNA Microarray Technology to Gerontological Studies
Sergey V. Anisimov
Detection and Analysis of Somatic Mutations at a LacZ Reporter Locus in Higher Organisms: Application to Mus Musculus and Drosophila Melanogaster
Ana Maria Garcia, Rita A. Busuttil, Armando Rodriguez, Carlos Cabrera, Martha Lundell, Martijn E. T. Dolle, and Jan Vijg
Subtractive Hybridization Techniques to Study Cellular Senescence
Michal B. Sternberg and Shimon Gepstein
Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity
Maria De Luca and Jeff Leips
Quantitative Trait Locus (QTL) Mapping in Aging Systems
Hui-Chen Hsu, Lu Lu, Nengjun Yi, Gary Van Zant, Robert W. Williams, and John D. Mountz
2D Gel Proteomics: An Approach to Study Age-related Differences in Protein-abundance or -Isoform Complexity in Biological Samples
Helen Kim, Shannon Eliuk, Jessy Deshane, Sreelatha Meleth, Todd Sanderson, Anita Pinner, Gloria Robinson, Landon Wilson, Marion Kirk, and Stephen Barnes
Metabolomics in the Study of Aging and Caloric Restriction
Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah- Daouk, and Wayne R. Matson
Index
Klappentext
Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. The editors have carefully selected only those topics that are considered mainstays of the field or are showing promise in revolutionizing this relatively new science. The three main areas of focus in this cutting-edge compendium of biological aging research are: methods that are basic to understanding the fundamental mechanisms of cellular aging; techniques used to intervene in the aging process; and approaches to analyzing the many molecular processes of biological aging. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions in the exciting and expanding field of biological aging.
New and novel techniques in biological aging research
Cutting-edge applications of proteomics and metabolomics
Identifying genes that extend lifespan using high-throughput screening systems
