Provides practical guides for the use of the reporter genes most commonly used in mammalian systems including b-galactosidase, GFP and luciferase
Easy to follow protocols for the use of reporter genes in various eukaryotic systems
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used.
Table of contentsPreface. Donald S. Anson.1. Methodologies for staining and visualisation of '-galactosidase inembryos and tissues. Siobhan Loughna* and Deborah Henderson.2. Immunohistochemical Detection of Beta-Galactosidase or GreenFluorescence Protein on Tissue Sections. Philip A. Seymour and MaikeSander*.3. Detection of Reporter Gene Expression in Murine Airways. MariaLimberis*, Peter Bell, and James M. Wilson.4. Three dimensional analysis of molecular signals with episcopicimaging techniques. Wolfgang J. Weninger* & Timothy J. Mohun5. Fluorescent proteins for cell biology. George H. Patterson*6. Detection of GFP during nervous system development in Drosophilamelanogaster. Karin Edoff, James S. Dods and Andrea H. Brand*7. Autofluorescent Proteins for Flow Cytometry. Charles G. Bailey andJohn E. J. Rasko*8. Fluorescent protein reporter systems for single cell measurements.Steven K. Dower*, Eva E. Qwarnstrom and Endre Kiss-Toth9. Subcellular Imaging of cancer cells in live mice. Robert M. Hoffman*10. Non-invasive Imaging of Molecular Events with BioluminescentReporter Genes in Living Subjects. Pritha Ray and Sanjiv Sam Gambhir*.11. Green Fluorescent Protein as a Tracer in Chimeric Tissues. HaraldJockusch* and Daniel Eberhard
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.
Methodologies for Staining and Visualisation of ?-Galactosidase in Mouse Embryos and Tissues.- Immunohistochemical Detection of ?-Galactosidase or Green Fluorescent Protein on Tissue Sections.- Detection of Reporter Gene Expression in Murine Airways.- Three-Dimensional Analysis of Molecular Signals with Episcopic Imaging Techniques.- Fluorescent Proteins for Cell Biology.- Detection of GFP During Nervous System Development in Drosophila melanogaster.- Autofluorescent Proteins for Flow Cytometry.- Fluorescent Protein Reporter Systems for Single-Cell Measurements.- Subcellular Imaging of Cancer Cells in Live Mice.- Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects.- Green Fluorescent Protein as a Tracer in Chimeric Tissues.
Inhaltsverzeichnis
Table of contents
Preface. Donald S. Anson.
1. Methodologies for staining and visualisation of '-galactosidase in
embryos and tissues. Siobhan Loughna* and Deborah Henderson.
2. Immunohistochemical Detection of Beta-Galactosidase or Green
Fluorescence Protein on Tissue Sections. Philip A. Seymour and Maike
Sander*.
3. Detection of Reporter Gene Expression in Murine Airways. Maria
Limberis*, Peter Bell, and James M. Wilson.
4. Three dimensional analysis of molecular signals with episcopic
imaging techniques. Wolfgang J. Weninger* & Timothy J. Mohun
5. Fluorescent proteins for cell biology. George H. Patterson*
6. Detection of GFP during nervous system development in Drosophila
melanogaster. Karin Edoff, James S. Dods and Andrea H. Brand*
7. Autofluorescent Proteins for Flow Cytometry. Charles G. Bailey and
John E. J. Rasko*
8. Fluorescent protein reporter systems for single cell measurements.
Steven K. Dower*, Eva E. Qwarnstrom and Endre Kiss-Toth
9. Subcellular Imaging of cancer cells in live mice. Robert M. Hoffman*
10. Non-invasive Imaging of Molecular Events with Bioluminescent
Reporter Genes in Living Subjects. Pritha Ray and Sanjiv Sam Gambhir*.
11. Green Fluorescent Protein as a Tracer in Chimeric Tissues. Harald
Jockusch* and Daniel Eberhard
Klappentext
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used.
Provides practical guides for the use of the reporter genes most commonly used in mammalian systems including b-galactosidase, GFP and luciferase
Easy to follow protocols for the use of reporter genes in various eukaryotic systems
