Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The authors' emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications.
Applications of Quantum Dots in Biology: An Overview Charles Z. Hotz Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots Ellen R. Goldman, Hedi Mattoussi, George P. Anderson, Igor L. Medintz, and J. Matthew Mauro Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates John N. Mason, Ian D. Tomlinson, Sandra J. Rosenthal, and Randy D. Blakely Peptide-Conjugated Quantum Dots: Imaging the Angiotensin Type 1 Receptor in Living Cells Ian D. Tomlinson, John N. Mason, Randy D. Blakely, and Sandra J. Rosenthal Quantum Dot-Encoded Beads Xiaohu Gao and Shuming Nie Use of Nanobarcodes® Particles in Bioassays R. Griffith Freeman, Paul A. Raju, Scott M. Norton, Ian D. Walton, Patrick C. Smith, Lin He, Michael J. Natan, Michael Y. Sha, and Sharron G. Penn Assembly and Characterization of Biomolecule-Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging Alexander Tkachenko, Huan Xie, Stefan Franzen, and Daniel L. Feldheim Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies Lee R. Hirsch, Naomi J. Halas, and Jennifer L. West Assays for Selection of Single-Chain Fragment Variable Recombinant Antibodies to Metal Nanoclusters Jennifer Edl, Ray Mernaugh, and David W. Wright Surface-Functionalized Nanoparticles for Controlled Drug Delivery Sung-Wook Choi, Woo-Sik Kim, and Jung-Hyun Kim Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis Joseph M. Slocik and David W. Wright Structural DNA Nanotechnology: An Overview Nadrian C. Seeman Nanostructured DNA Templates Jeffery L. Coffer, Russell F. Pinizzotto, and Young Gyu Rho Probing DNA Structure With Nanoparticles Rahina Mahtab and Catherine J. Murphy Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells TimothyE. McKnight, Anatoli V. Melechko, Michael A. Guillorn, Vladimir I. Merkulov, Douglas H. Lowndes, and Michael L. Simpson Real-Time Cell Dynamics With a Multianalyte Physiometer Sven E. Eklund, Eugene Kozlov, Dale E. Taylor, Franz Baudenbacher, and David E. Cliffel Index
The combination of nanoscience and biotechnology promises to yield revolutionary biological insights, ranging from receptor function to drug discovery to personal medicine. In NanoBiotechnology Protocols, hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The authors emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used as a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotechnology arena. A value-added compact disk containing color figures is included. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Illuminating and cross-disciplinary, NanoBiotechnology Protocols enables novice and experienced researchers alike to quickly come up to speed with both nanomaterials preparation and the use of nanomaterials in biological and medicinal applications.
Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.
Applications of Quantum Dots in Biology.- Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots.- Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates.- Peptide-Conjugated Quantum Dots.- Quantum Dot-Encoded Beads.- Use of Nanobarcodes® Particles in Bioassays.- Assembly and Characterization of Biomolecule-Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging.- Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies.- Assays for Selection of Single-Chain Fragment Variable Recombinant Antibodies to Metal Nanoclusters.- Surface-Functionalized Nanoparticles for Controlled Drug Delivery.- Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis.- Structural DNA Nanotechnology.- Nanostructured DNA Templates.- Probing DNA Structure With Nanoparticles.- Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells.- Real-Time Cell Dynamics With a Multianalyte Physiometer.
Inhaltsverzeichnis
Applications of Quantum Dots in Biology: An Overview
Charles Z. Hotz
Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots
Ellen R. Goldman, Hedi Mattoussi, George P. Anderson, Igor L. Medintz, and J. Matthew Mauro
Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates
John N. Mason, Ian D. Tomlinson, Sandra J. Rosenthal, and Randy D. Blakely
Peptide-Conjugated Quantum Dots: Imaging the Angiotensin Type 1 Receptor in Living Cells
Ian D. Tomlinson, John N. Mason, Randy D. Blakely, and Sandra J. Rosenthal
Quantum Dot-Encoded Beads
Xiaohu Gao and Shuming Nie
Use of Nanobarcodes® Particles in Bioassays
R. Griffith Freeman, Paul A. Raju, Scott M. Norton, Ian D. Walton, Patrick C. Smith, Lin He, Michael J. Natan, Michael Y. Sha, and Sharron G. Penn
Assembly and Characterization of Biomolecule-Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging
Alexander Tkachenko, Huan Xie, Stefan Franzen, and Daniel L. Feldheim
Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies
Lee R. Hirsch, Naomi J. Halas, and Jennifer L. West
Assays for Selection of Single-Chain Fragment Variable Recombinant Antibodies to Metal Nanoclusters
Jennifer Edl, Ray Mernaugh, and David W. Wright
Surface-Functionalized Nanoparticles for Controlled Drug Delivery
Sung-Wook Choi, Woo-Sik Kim, and Jung-Hyun Kim
Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis
Joseph M. Slocik and David W. Wright
Structural DNA Nanotechnology: An Overview
Nadrian C. Seeman
Nanostructured DNA Templates
Jeffery L. Coffer, Russell F. Pinizzotto, and Young Gyu Rho
Probing DNA Structure With Nanoparticles
Rahina Mahtab and Catherine J. Murphy
Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells
TimothyE. McKnight, Anatoli V. Melechko, Michael A. Guillorn, Vladimir I. Merkulov, Douglas H. Lowndes, and Michael L. Simpson
Real-Time Cell Dynamics With a Multianalyte Physiometer
Sven E. Eklund, Eugene Kozlov, Dale E. Taylor, Franz Baudenbacher, and David E. Cliffel
Index
Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The authors' emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications.
