Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume provides an overview of modern and emerging methods for production, analysis, and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Peptide Libraries: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.
I. Chemical Strategies for Library Synthesis
1. Design, Synthesis and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries
Kit Lam and Ruiwu Liu
2. Synthesis of Macrocyclic Organo-Peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization
Jessica M. Smith and Rudi Fasan
3. Synthesis and Screening of One-Bead-One-Compound Cyclic Peptide Libraries
Ziqing Qian, Punit Upadhyaya, and Dehua Pei
4. Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation
Kristen A. Andersen and Ronald T. Raines
5. Macrocyclic Templates for Library Synthesis of Peptido-Conjugates
Serge Zaretsky, Joanne Tan, Jennifer L. Hickey, and Andrei K. Yudin
6. Synthesis and Alkylation of aza-Gly-Pro Building Blocks of Peptidomimetic Libraries for Developing Prostaglandin F2? Receptor Modulators as Therapeutics to Inhibit Preterm Labour
Carine B. Bourguet and William D. Lubell
II. Chemistry and Genetically-Encoded Peptide Libraries
7. A Fragment Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases
Elizabeth Restituyo, Karla Camacho-Soto, and Indraneel Ghosh
8. Peptide and Peptide Library Cyclization Via Bromomethyl Benzene Derivatives
David E. Hacker, Mohammed Almohaini, Aruna Anbazhagan, Zhong Ma, and Matthew C. T. Hartman
9. Phage Selection of Bicyclic Peptides Based on Two Disulfide Bridges
Shiyu Chen and Christian Heinis
10. De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry
Amol V. Shivange and Patrick S. Daugherty
11. Chemical Post-Translational Modification of Phage-Displayed Peptides
Simon Ng, Katrina F. Tjhung, Beth M. Paschal, Christopher J. Noren, and Ratmir Derda
12. Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries and Alanine-Scanning
Malgorzata E. Kokoszka and Brian K. Kay
13. Identifying Reactive Peptides from Phage-Displayed Libraries
Glenn M. Eldridge and Gregory A. Weiss
14. The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding Tags
Langdon J. Martin and Barbara Imperiali
III. Utility of Peptide Libraries
15. Synthesis and Cell-Based Screening of One-Bead One-Compound Peptide Libraries
F.C. Bononi and Len G. Luyt
16. Screening Peptide Array Library for the Identification of Cancer Cell Binding Peptides
Kamaljit Kaur, Sahar Ahmed, Rania Soudy, and Sarfuddin Azmi
17. Next Generation Sequencing of Phage-Displayed Peptide Libraries
Ratmir Derda and Wadim Matochko
18. Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries
Buyung Santoso and Brion W. Murray
This volume provides an overview of modern and emerging methods for production, analysis, and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Peptide Libraries: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.
Design, Synthesis and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries.- Synthesis of Macrocyclic Organo-Peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization.- Synthesis and Screening of One-Bead-One-Compound Cyclic Peptide Libraries.- Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation.- Macrocyclic Templates for Library Synthesis of Peptido-Conjugates.- Synthesis and Alkylation of aza-Gly-Pro Building Blocks of Peptidomimetic Libraries for Developing Prostaglandin F2 Receptor Modulators as Therapeutics to Inhibit Preterm Labour.- A Fragment Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases.- Peptide and Peptide Library Cyclization Via Bromomethyl Benzene Derivatives.- Phage Selection of Bicyclic Peptides Based on Two Disulfide Bridges.- De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry.- Chemical Post-Translational Modification of Phage-Displayed Peptides.- Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries and Alanine-Scanning.- Identifying Reactive Peptides from Phage-Displayed Libraries.- The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding Tags.- Synthesis and Cell-Based Screening of One-Bead One-Compound Peptide Libraries.- Screening Peptide Array Library for the Identification of Cancer Cell Binding Peptides.- Next Generation Sequencing of Phage-Displayed Peptide Libraries.- Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries.
Inhaltsverzeichnis
I. Chemical Strategies for Library Synthesis
1. Design, Synthesis and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries
Kit Lam and Ruiwu Liu
2. Synthesis of Macrocyclic Organo-Peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization
Jessica M. Smith and Rudi Fasan
3. Synthesis and Screening of One-Bead-One-Compound Cyclic Peptide Libraries
Ziqing Qian, Punit Upadhyaya, and Dehua Pei
4. Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation
Kristen A. Andersen and Ronald T. Raines
5. Macrocyclic Templates for Library Synthesis of Peptido-Conjugates
Serge Zaretsky, Joanne Tan, Jennifer L. Hickey, and Andrei K. Yudin
6. Synthesis and Alkylation of aza-Gly-Pro Building Blocks of Peptidomimetic Libraries for Developing Prostaglandin F2a Receptor Modulators as Therapeutics to Inhibit Preterm Labour
Carine B. Bourguet and William D. Lubell
II. Chemistry and Genetically-Encoded Peptide Libraries
7. A Fragment Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases
Elizabeth Restituyo, Karla Camacho-Soto, and Indraneel Ghosh
8. Peptide and Peptide Library Cyclization Via Bromomethyl Benzene Derivatives
David E. Hacker, Mohammed Almohaini, Aruna Anbazhagan, Zhong Ma, and Matthew C. T. Hartman
9. Phage Selection of Bicyclic Peptides Based on Two Disulfide Bridges
Shiyu Chen and Christian Heinis
10. De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry
Amol V. Shivange and Patrick S. Daugherty
11. Chemical Post-Translational Modification of Phage-Displayed Peptides
Simon Ng, Katrina F. Tjhung, Beth M. Paschal, Christopher J. Noren, and Ratmir Derda
12. Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries and Alanine-Scanning
Malgorzata E. Kokoszka and Brian K. Kay
13. Identifying Reactive Peptides from Phage-Displayed Libraries
Glenn M. Eldridge and Gregory A. Weiss
14. The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding Tags
Langdon J. Martin and Barbara Imperiali
III. Utility of Peptide Libraries
15. Synthesis and Cell-Based Screening of One-Bead One-Compound Peptide Libraries
F.C. Bononi and Len G. Luyt
16. Screening Peptide Array Library for the Identification of Cancer Cell Binding Peptides
Kamaljit Kaur, Sahar Ahmed, Rania Soudy, and Sarfuddin Azmi
17. Next Generation Sequencing of Phage-Displayed Peptide Libraries
Ratmir Derda and Wadim Matochko
18. Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries
Buyung Santoso and Brion W. Murray
Klappentext
This volume provides an overview of modern and emerging methods for production, analysis, and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nAuthoritative and practical, Peptide Libraries: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
