The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in tranillegalscription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select "Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.
Part I. Gene Expression Control at the mRNA LevelJohn P. Vanden HeuvelChapter 1. OverviewChapter 2. Messenger RNA AccumulationChapter 3. Tranillegalscript ProfilingChapter 4. Tranillegalscriptional Regulation of Gene ExpressionChapter 5. Posttranillegalscriptional Processing of Messenger RNAStudy QuestionsReferencesPart II. Regulation of Protein Levels and Tranillegalscription Factor FunctionGary H. PerdewChapter 1. OverviewChapter 2. Protein Synthesis and TurnoverChapter 3. ProteomicsChapter 4. Determination of Protein-Protein Interactions and the Motifs That Mediate ThemChapter 5. Posttranslational ModificationsChapter 6. Dissection of Signaling PathwaysStudy QuestionsReferencesPart III. Use of Transgenic and Knockout Mice to Study Gene RegulationJeffrey M. PetersChapter 1. OverviewChapter 2. Production of Transgenic AnimalsChapter 3. Knockout AnimalsChapter 4. Isolation of Cells and Cell Lines From Transgenic and Knockout AnimalsStudy QuestionsReferencesIndex
Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein.
Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given tranillegalscription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-protein interactions, and posttranslational modification. Part III explores how gene targeting techniques in mice can provide insight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediated alterations in gene expression in mammalian systems.
From the reviews:
"The book is aimed at graduate students, filling a gap between more general text books ... and laboratory manuals (e.g. the Current Protocols series). ... Helpful notes and key references are given in the margins throughout the text. ... overall `Regulation of Gene Expression: Molecular Mechanisms´ would certainly be of use to life sciences undergraduates, it would also be a useful reference work for a Molecular Toxicology laboratory." (James Sidaway, British Toxicology Society Newsletter, Summer, 2007)
The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in tranillegalscription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit cas. psu. edu and select "Courses. " The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.
Gene Expression Control At The Mrna Level.- Overview.- Messenger RNA Accumulation.- Tranillegalscript Profiling.- Tranillegalscriptional Regulation of Gene Expression.- Posttranillegalscriptional Processing of Messenger RNA.- Regulation Of Protein Levels And Tranillegalscription Factor Function.- Overview.- Protein Synthesis and Turnover.- Proteomics.- Determination of Protein-Protein Interactions and the Motifs That Mediate Them.- Posttranslational Modifications.- Dissection of Signaling Pathways.- Use Of Transgenic And Knockout Mice To Study Gene Regulation.- Overview.- Production of Transgenic Animals.- Knockout Animals.- Isolation of Cells and Cell Lines From Transgenic and Knockout Animals.
From the reviews:
"The book is aimed at graduate students, filling a gap between more general text books ... and laboratory manuals (e.g. the Current Protocols series). ... Helpful notes and key references are given in the margins throughout the text. ... overall 'Regulation of Gene Expression: Molecular Mechanisms' would certainly be of use to life sciences undergraduates, it would also be a useful reference work for a Molecular Toxicology laboratory." (James Sidaway, British Toxicology Society Newsletter, Summer, 2007)
Inhaltsverzeichnis
Part I. Gene Expression Control at the mRNA Level
John P. Vanden Heuvel
Chapter 1. Overview
Chapter 2. Messenger RNA Accumulation
Chapter 3. Tranillegalscript Profiling
Chapter 4. Tranillegalscriptional Regulation of Gene Expression
Chapter 5. Posttranillegalscriptional Processing of Messenger RNA
Study Questions
References
Part II. Regulation of Protein Levels and Tranillegalscription Factor Function
Gary H. Perdew
Chapter 1. Overview
Chapter 2. Protein Synthesis and Turnover
Chapter 3. Proteomics
Chapter 4. Determination of Protein-Protein Interactions and the Motifs That Mediate Them
Chapter 5. Posttranslational Modifications
Chapter 6. Dissection of Signaling Pathways
Study Questions
References
Part III. Use of Transgenic and Knockout Mice to Study Gene Regulation
Jeffrey M. Peters
Chapter 1. Overview
Chapter 2. Production of Transgenic Animals
Chapter 3. Knockout Animals
Chapter 4. Isolation of Cells and Cell Lines From Transgenic and Knockout Animals
Study Questions
References
Index
Klappentext
The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in tranillegalscription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select ¿Courses. ¿ The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.
Includes supplementary material: sn.pub/extras
